Beta-Hydroxy-Beta-Methylbutyrate or Calcium HMB is a metabolite of the essential amino acid leucine. It is naturally produced in human body and small quantities can also be found in grapefruit, alfalfa, and catfish. Calcium HMB is used as a supplement because research has shown it to Increase sarcolemmal integrity via conversion to HMG-CoA and to enhance protein synthesis. It also helps in depression of protein degradation through inhibition of the ubiquitin pathway. Calcium HMB is proved to help muscle formulation so it is given to patients with HIV or AIDS and trauma victims with severe injuries. It is also used in treatment of cancer.

Creatine Monohydrate is also called methyl guanidine-acetic acid. It is a naturally occurring substance in the muscle cells particularly in the skeletal muscle. It is a metabolite that consists of three amino acids, namely: methionine, arginine and glycine. Creatine Monohydrate helps produce and supply more energy to the muscles. It is converted in the muscles into phosphocreatine that generates Adenosine triphosphate or ATP that is responsible for increasing its energy and strength. It also provides burst of high-intensity energy because of the increase in ATP. It also increases the endurance of the muscles that allows working out for a longer period – more repetitions would result in bigger, stronger and firmer muscles.

L-Arginine is an amino acid that helps the body get rid of ammonia and is used to make compounds such as creatine, l-proline and l-glutamine which is then converted into glucose as the body needs it.

L-Citrulline is an organic compound and is an alpha amino acid. It is also a non-essential amino acid meaning it is synthesized within the human body itself. L-Citrulline is a primary element in the urea cycle which is the process by which mammals excrete ammonia. This is done in the mitochondria of cells where ammonia combines with carbon dioxide and ornithine to form citrulline. This Citrulline is carried out to the cytoplasm where it is converted to arginine. This way the ammonia is removed from muscle and liver cells. L-Citrulline s found in high concentrations in the liver and while it is not a component of most proteins found in the body, L-Citrulline is found in specialised proteins in the hair, skin and neural cells. Within the body Lcitrulline is mainly synthesized from glutamine in the intestine.

L-Glycine- Glycine is an organic compound and one of the 20 amino acids deemed as building blocks for proteins. This proteinogenic amino acid is not only the smallest but also the only amino acid of the group that is not chiral, which means there is no enantiomer of glycine. With the prominent exception of collagen, which thirty five percent are glycine, most proteins contain only small amounts of it. As a solid, glycine is sweet tasting, crystalline and has no color. Despite being non-essential, because it can be biosynthesized from the amino acid serine, glycine has several important physiological functions, besides being a precursor to proteins. It is a biosynthetic intermediate of purines and the D-Aminolevulinic acid and it is an inhibitory neurotransmitter found in the central nervous system.

L-Ornithine is produced when the enzyme arginase reacts on L-arginine, whereby urea is being created. Consequentially, ornithine seems to be a vital part of the urea cycle, the disposal of ammonia and the removal of excess nitrogen. Although ornithine is not coded for by the DNA, which means that it is not a part of the protein synthesis processes. Despite this fact, the urea cycle plays a big part in arginine biosynthesis, which means that as an intermediate in a metabolic process, ornithine still retains vital importance. L-Ornithine is gaining on popularity as a nutritional supplement, in particular with the body building circles, where it is propagated as a muscle building and fat loss agent. It is furthermore stated that L-Ornithine may be initiating the release of growth hormones in the body.